![]() © 2012 International Society for Neurochemistry. Here, we will revise the latest findings on the characterization of active and quiescent adult neural stem cells in the main regions of neurogenesis and the factors necessary to maintain their active and resting states, stimulate migration and homing in diseased areas, hoping to outline the emerging knowledge for the promotion of regeneration in the brain based on endogenous stem cells. In the nervous system, during development, niches control neural stem cells (NSCs) maturation and the formation of the neuronal network. In particular, a promising field is aimed at stimulating or trigger a self-repair system in the diseased brain driven by its own stem cell population. Adult neural stem cells have arisen considerable interest as these studies can be useful to develop new methods to replace damaged neurons and treat severe neurological diseases such as neurodegeneration, stroke or spinal cord lesions. Nevertheless, many reports indicate that they subsist in other regions of the adult brain. We previously described direct reprogramming of adult mouse neural stem cells (NSCs) by Oct4 and either Klf4 or c-Myc. They are capable of forming multipotent neurospheres when cultured in vitro. The four transcription factors Oct4, Sox2, Klf4, and c-Myc can induce pluripotency in mouse and human fibroblasts. Neural stem cells in the adult mammalian brain are located in the 'neurogenic' areas of the subventricular and subgranular zones. Adult NSCs were first isolated from mouse striatum in the early 1990s. Whether working with mature neuronal cells from the brain or neural stem cells (NSCs) obtained from the subventricular zone (SVZ) of the forebrain. In the beginning of the 1990s, neurogenesis in the adult brain was indisputably accepted, eliciting great research efforts. Adult somatic stem cells in various organs maintain homeostatic tissue regeneration and enhance plasticity. PMCID: PMC8121769 DOI: 10.1016/j.xpro.2021.100522 Abstract This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Neural stem cells are also affected in some brain diseases, such as Parkinson’s and Alzheimer’s. In adults, these cells enter a dormant state. They show that interfering with oleic acid signaling or synthesis rescues NSC proliferation in a mouse model of Alzheimer’s, revealing a potential approach to promote NSC-mediated brain function and repair. Research on stem cells has developed as one of the most promising areas of neurobiology. The mechanisms explaining how and why stem cells help recovery from brain injury are an important and active area of research. Collectively, these data emphasize an important postnatal role for the cells of the central canal region. identify deregulation of niche fatty acid metabolism as a mechanism of disease-induced stem cell impairment.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |